Mouse Anti-TNFSF4 Recombinant Antibody (clone R4930) (CAT#: NEUT-2204CQ)
This antibody is a mouse monoclonal antibody that binds specifically to OX40L, and it can neutralize the bioactivity of OX40L.
Specific Inquiry
SDS-PAGE
Figure 1 Mouse Anti-TNFSF4 Recombinant Antibody (NEUT-2204CQ) in SDS-PAGE
SDS-PAGE analysis of NEUT-2204CQ in non-reduced (Lane 1) and reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
DB
Figure 2 Mouse Anti-TNFSF4 Recombinant Antibody (NEUT-2204CQ) in DB
Dot blot analysis of NEUT-2204CQ was performed by loading Human OX40 Ligand / TNFSF4 Protein, active trimer (His Tag).
NEUT-2204CQ incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Anti-Mouse IgG (H+L)
WB
Figure 3 Mouse Anti-TNFSF4 Recombinant Antibody (NEUT-2204CQ) in WB
Western blot analysis of NEUT-2204CQ was performed by loading Human OX40 Ligand / TNFSF4 Protein, active trimer (His Tag).
NEUT-2204CQ incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Anti-Mouse IgG (H+L)
Lane 1: Reducing antigen (0.1 μg)
Lane 2: Reducing antigen (0.3 μg)
Lane 3: Reducing antigen (0.6 μg)
ELISA
Figure 4 Mouse Anti-TNFSF4 Recombinant Antibody (NEUT-2204CQ) in ELISA
ELISA analysis of NEUT-2204CQ was performed by coating with Human OX40 Ligand / TNFSF4 Protein, active trimer (His tag).
The secondary antibody: HRP-Anti-Mouse IgG (H+L)
Specifications
- Immunogen
- Human OX40L, the ligand of OX40
- Host Species
- Mouse
- Type
- Mouse IgG, κ
- Specificity
- Human TNFSF4
- Species Reactivity
- Human
- Clone
- R4930
- Applications
- FC, Block
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- TNFSF4; TNF superfamily member 4; GP34; CD252; OX4OL; TXGP1; CD134L; OX-40L; TNLG2B
- Gene ID
- 7292
- UniProt ID
- P23510
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Flow Cytometry (FC) related Reagents and Kits: Empowering Cell Function Research
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1645z | Mouse Anti-TNFSF4 Recombinant Antibody (clone 28F11) | FC, IHC, Neut | Mouse IgG1 |
| MOB-0429ZL | Mouse Anti-TNFSF4 Recombinant Antibody (clone YC164) | Neut, FC, IHC | Mouse IgG1 |
| HPAB-0144-CN | Mouse Anti-TNFSF4 Recombinant Antibody (HPAB-0144-CN) | ELISA, FC | Mouse IgG |
| HPAB-0145-CN | Mouse Anti-TNFSF4 Recombinant Antibody (HPAB-0145-CN) | ELISA, FC | Mouse IgG |
| HPAB-0146-CN | Human Anti-TNFSF4 Recombinant Antibody (HPAB-0146-CN) | ELISA, FC | Human IgG1, κ |
Customer Reviews and Q&As
Customer Review
Q&As
High Sensitivity in Detection
We have observed remarkable sensitivity in TNFSF4 detection, facilitating the precise quantification of expression levels across different conditions. This sensitivity is crucial for the subtle changes we are investigating.
Great Performance in Functional Assays
The utility of the Anti-TNFSF4 antibody in functional assays has been instrumental, allowing us to dissect complex signaling pathways during immune responses. Its consistent performance enhances the credibility of our findings.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# NEUT-2204CQ, RRID: AB_3111565)
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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